ARG62953
anti-CD97 antibody [MEM-180]
anti-CD97 antibody [MEM-180] for Flow cytometry,Immunoprecipitation and Human
Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-180] recognizes CD97 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP |
| Specificity | The clone MEM-180 recognizes an unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes. HLDA VI; WS Code BP 415 HLDA VI; WS Code NL N-L023 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-180 |
| Isotype | IgG1 |
| Target Name | CD97 |
| Immunogen | PHA-activated peripheral blood cells |
| Conjugation | Un-conjugated |
| Alternate Names | CD97; Leukocyte antigen CD97; CD97 antigen; CD antigen CD97; TM7LN1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ADGRE5 |
| Gene Full Name | adhesion G protein-coupled receptor E5 |
| Background | CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis. |
| Function | Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity). [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 92 kDa |
| PTM | Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. |
