ARG62954

anti-CD97 antibody [MEM-180] (FITC)

anti-CD97 antibody [MEM-180] (FITC) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description

FITC-conjugated Mouse Monoclonal antibody [MEM-180] recognizes CD97

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-180 recognizes an unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.
HLDA VI; WS Code BP 415
HLDA VI; WS Code NL N-L023
Host Mouse
Clonality Monoclonal
Clone MEM-180
Isotype IgG1
Target Name CD97
Immunogen PHA-activated peripheral blood cells
Conjugation FITC
Alternate Names CD97; Leukocyte antigen CD97; CD97 antigen; CD antigen CD97; TM7LN1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 976 Human ADGRE5

Swiss-port # P48960 Human CD97 antigen

Gene Symbol ADGRE5
Gene Full Name adhesion G protein-coupled receptor E5
Background CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
Function Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity). [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 92 kDa
PTM Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.