ARG41845

anti-CDC16 / APC6 antibody

anti-CDC16 / APC6 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes CDC16 / APC6
Tested Reactivity Hu
Predict Reactivity Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CDC16 / APC6
Antigen Species Human
Immunogen An 18 amino acid synthetic peptide within the first 50 amino acids of Human CDC16 / APC6.
Conjugation Un-conjugated
Alternate Names Cell division cycle protein 16 homolog; Cyclosome subunit 6; Anaphase-promoting complex subunit 6; CDC16Hs; CDC16 homolog; APC6; CUT9; ANAPC6

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 - 20 µg/ml
WB1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human liver
Observed Size ~ 72 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8881 Human CDC16

Swiss-port # Q13042 Human Cell division cycle protein 16 homolog

Gene Symbol CDC16
Gene Full Name cell division cycle 16
Background This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. [UniProt]
Calculated MW 72 kDa
PTM Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis. [UniProt]