ARG41611

anti-CDC27 antibody

anti-CDC27 antibody for Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CDC27
Tested Reactivity Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CDC27
Antigen Species Human
Immunogen A 19 amino acid peptide within the last 50 amino acids of Human CDC27.
Conjugation Un-conjugated
Alternate Names HNUC; Cell division cycle protein 27 homolog; CDC27 homolog; NUC2; D0S1430E; H-NUC; Anaphase-promoting complex subunit 3; CDC27Hs; APC3; ANAPC3; D17S978E

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 217232 Mouse CDC27

Swiss-port # A2A6Q5 Mouse Cell division cycle protein 27 homolog

Gene Symbol CDC27
Gene Full Name cell division cycle 27
Background The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 92 kDa
PTM Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. [UniProt]