ARG52839
anti-CDX2 antibody [SP54]
anti-CDX2 antibody [SP54] for IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP54] recognizes CDX2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP54 |
| Isotype | IgG |
| Target Name | CDX2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to N-terminus of human CDX2. |
| Conjugation | Un-conjugated |
| Alternate Names | CDX2/AS; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Colon Carcinoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | CDX2 is a caudal type homeobox gene that encodes an intenstine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared in CK20. |
| Cellular Localization | Nucleus |
| Highlight | Related products: CDX2 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Wnt / beta-catenin signaling for gastric fundus specification |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 34 kDa |
| PTM | Phosphorylation of Ser-60 mediates the transactivation capacity. |
Clone References
5-Aza-dC treatment induces mesenchymal-to-epithelial transition in 1st trimester trophoblast cell line HTR8/SVneo.
ICC/IF / Human
