ARG62441

anti-CDw75 / ST6GAL1 antibody [LN1]

anti-CDw75 / ST6GAL1 antibody [LN1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [LN1] recognizes CDw75 / ST6GAL1
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Specificity Reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin’s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
Host Mouse
Clonality Monoclonal
Clone LN1
Isotype IgM
Target Name CDw75 / ST6GAL1
Antigen Species Human
Immunogen Tissue, cells or virus corresponding to Human CDw75. Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
Conjugation Un-conjugated
Alternate Names SIAT1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; B-cell antigen CD75; Sialyltransferase 1; ST6Gal I; Beta-galactoside alpha-2,6-sialyltransferase 1; EC 2.4.99.1; ST6N; Alpha 2,6-ST 1; ST6GalI

Application Instructions

Application Suggestion
Tested Application Dilution
FACS5 - 10 µl/10^6 cells
ICC/IF1:25 - 1:50
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6480 Human ST6GAL1

Swiss-port # P15907 Human Beta-galactoside alpha-2,6-sialyltransferase 1

Gene Symbol ST6GAL1
Gene Full Name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Background This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009]
Function Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates. [UniProt]
Cellular Localization Golgi Apparatus; Golgi stack; Golgi stack membrane; single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi. Secreted protein; body fluid.
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 47 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.