ARG56205

anti-CELA3B / Elastase 3B antibody [ELTS3B-2]

anti-CELA3B / Elastase 3B antibody [ELTS3B-2] for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [ELTS3B-2] recognizes CELA3B / Elastase 3B
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P
Host Mouse
Clonality Monoclonal
Clone ELTS3B-2
Isotype IgG
Target Name CELA3B / Elastase 3B
Antigen Species Human
Immunogen A partial recombinant Elastase 3B / ELA3B protein (aa 82-238).
Conjugation Un-conjugated
Alternate Names ELA3B; CBPP; EC 3.4.21.70; Elastase-3B; Chymotrypsin-like elastase family member 3B; Elastase IIIB; EL-1; Protease E; E1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23436 Human CELA3B

GeneID: 67868 Mouse CELA3B

Swiss-port # P08861 Human Chymotrypsin-like elastase family member 3B

Swiss-port # Q9CQ52 Mouse Chymotrypsin-like elastase family member 3B

Gene Symbol CELA3B
Gene Full Name chymotrypsin-like elastase family, member 3B
Background Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009]
Function Efficient protease with alanine specificity but only little elastolytic activity. [UniProt]
Calculated MW 29 kDa