ARG66605

anti-CEP135 antibody

anti-CEP135 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CEP135
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CEP135
Antigen Species Human
Immunogen Synthetic peptide within aa. 1050-1130 of Human CEP135.
Conjugation Un-conjugated
Alternate Names Centrosomal protein of 135 kDa; KIAA0635; MCPH8; CEP4; Cep135; Centrosomal protein 4

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P1:100 - 1:300
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: High-pressure and temperature Tris-EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 130 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 381644 Mouse CEP135

GeneID: 9662 Human CEP135

Swiss-port # Q66GS9 Human Centrosomal protein of 135 kDa

Swiss-port # Q6P5D4 Mouse Centrosomal protein of 135 kDa

Gene Symbol CEP135
Gene Full Name centrosomal protein 135kDa
Background This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012]
Function Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles. [UniProt]
Calculated MW 133 kDa