ARG41829
anti-CHD3 antibody
anti-CHD3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Flow cytometry and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CHD3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHD3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human CHD3. |
| Conjugation | Un-conjugated |
| Alternate Names | Zinc finger helicase; Mi2-ALPHA; Chromodomain-helicase-DNA-binding protein 3; hZFH; Mi2-alpha; Mi-2a; Mi-2 autoantigen 240 kDa protein; ZFH; EC 3.6.4.12; ATP-dependent helicase CHD3; CHD-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | K562 | ||||||||||
| Observed Size | ~ 250 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q12873 Human Chromodomain-helicase-DNA-binding protein 3 |
|---|---|
| Gene Symbol | CHD3 |
| Gene Full Name | chromodomain helicase DNA binding protein 3 |
| Background | This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Function | Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase and mitosis. [UniProt] |
| Calculated MW | 227 kDa |
