ARG63479

anti-CHFR antibody

anti-CHFR antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes CHFR
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Pig
Tested Application IHC-P
Specificity This antibody is expected to recognise all reported isoforms.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CHFR
Antigen Species Human
Immunogen KFNHICEQTRFKN
Conjugation Un-conjugated
Alternate Names EC 6.3.2.-; RNF196; E3 ubiquitin-protein ligase CHFR; RNF116; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55743 Human CHFR

Swiss-port # Q96EP1 Human E3 ubiquitin-protein ligase CHFR

Background This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 73 kDa
PTM Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
Autoubiquitinated; may regulate its cellular level.
Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.