ARG40721
anti-CHMP5 antibody
anti-CHMP5 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CHMP5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHMP5 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to aa. 1-219 of Human CHMP5. |
| Conjugation | Un-conjugated |
| Alternate Names | hVps60; Charged multivesicular body protein 5; SNF7 domain-containing protein 2; HSPC177; CGI-34; Vps60; PNAS-2; Chromatin-modifying protein 5; Vacuolar protein sorting-associated protein 60; SNF7DC2; C9orf83 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 25 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Caprylic acid ammonium sulfate precipitation. |
| Buffer | 0.01M PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol. |
| Preservative | 0.03% Proclin 300 |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NZZ3 Human Charged multivesicular body protein 5 |
|---|---|
| Gene Symbol | CHMP5 |
| Gene Full Name | charged multivesicular body protein 5 |
| Background | CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008] |
| Function | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein. Note=Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | ISGylated. Isgylation inhibits its interaction with VTA1. [UniProt] |
