ARG41767

anti-CHRM2 antibody

anti-CHRM2 antibody for IHC-Formalin-fixed paraffin-embedded sections and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CHRM2
Tested Reactivity Rat
Predict Reactivity Hm
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CHRM2
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 356-370 of Human CHRM2. (EKQNIVARKIVKMTK)
Conjugation Un-conjugated
Alternate Names Muscarinic acetylcholine receptor M2; HM2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CHRM2
Gene Full Name cholinergic receptor, muscarinic 2
Background The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol. [UniProt]
Cellular Localization Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note=Phosphorylation in response to agonist binding promotes receptor internalization. [UniProt]
Calculated MW 52 kDa
PTM Phosphorylated in response to agonist treatment. [UniProt]