ARG64681

anti-CHRNA4 antibody

anti-CHRNA4 antibody for Western blot and Human,Rat

Cancer antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes CHRNA4
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Cow, Dog
Tested Application WB
Specificity This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CHRNA4
Antigen Species Human
Immunogen C-HVETRAHAEERLLKK
Conjugation Un-conjugated
Alternate Names Neuronal acetylcholine receptor subunit alpha-4; NACRA4; NACHRA4; EBN; BFNC; EBN1; NACHR

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1137 Human CHRNA4

GeneID: 25590 Rat CHRNA4

Swiss-port # P09483 Rat Neuronal acetylcholine receptor subunit alpha-4

Swiss-port # P43681 Human Neuronal acetylcholine receptor subunit alpha-4

Background This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Research Area Cancer antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 70 kDa