ARG58437

anti-CHRNE antibody

anti-CHRNE antibody for Western blot and Mouse

Overview

Product Description Goat Polyclonal antibody recognizes CHRNE
Tested Reactivity Ms
Predict Reactivity Hu, Rat, Cow
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CHRNE
Antigen Species Human
Immunogen Synthetic peptide from the internal region of Human CHRNE (NP_000071.1). (C-DQEATGEEVSD)
Conjugation Un-conjugated
Alternate Names FCCMS; CMS1E; CMS1D; SCCMS; CMS2A; Acetylcholine receptor subunit epsilon; CMS4B; CMS4C; ACHRE; CMS4A

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 55 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11448 Mouse CHRNE

Swiss-port # P20782 Mouse Acetylcholine receptor subunit epsilon

Gene Symbol CHRNE
Gene Full Name cholinergic receptor, nicotinic, epsilon (muscle)
Background Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009]
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. [UniProt]
Calculated MW 55 kDa