ARG42039
anti-CLASP1 antibody [KT66]
anti-CLASP1 antibody [KT66] for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rat Monoclonal antibody [KT66] recognizes CLASP1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | KT66 |
| Isotype | IgG2a |
| Target Name | CLASP1 |
| Antigen Species | Mouse |
| Immunogen | GST fusion protein around the C-terminus of Mouse CLASP1. |
| Conjugation | Un-conjugated |
| Alternate Names | Multiple asters homolog 1; Protein Orbit homolog 1; MAST1; CLIP-associating protein 1; hOrbit1; Cytoplasmic linker-associated protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 161 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLASP1 |
| Gene Full Name | cytoplasmic linker associated protein 1 |
| Background | CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008] |
| Function | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton, spindle, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Golgi apparatus, trans-Golgi network. Note=Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. [UniProt] |
| Calculated MW | 169 kDa |
