ARG42040
anti-CLASP2 antibody [KT68]
anti-CLASP2 antibody [KT68] for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rat Monoclonal antibody [KT68] recognizes CLASP2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | KT68 |
| Isotype | IgG2b |
| Target Name | CLASP2 |
| Antigen Species | Mouse |
| Immunogen | GST fusion protein around the N-terminus of Mouse CLASP2. |
| Conjugation | Un-conjugated |
| Alternate Names | Cytoplasmic linker-associated protein 2; Protein Orbit homolog 2; hOrbit2; CLIP-associating protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLASP2 |
| Gene Full Name | cytoplasmic linker associated protein 2 |
| Function | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton, spindle, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Golgi apparatus, trans-Golgi network. Cell membrane. Cell projection, ruffle membrane. [UniProt] |
| Calculated MW | 141 kDa |
| PTM | Phosphorylated by GSK3B. Phosphorylation reduces MAPRE1 binding (PubMed:26003921). Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella. [UniProt] |
