ARG22589

anti-CLEC2 / CLEC1B antibody [17D9] (FITC)

anti-CLEC2 / CLEC1B antibody [17D9] (FITC) for Flow cytometry and Mouse

Overview

Product Description FITC-conjugated Rat Monoclonal antibody [17D9] recognizes CLEC2 / CLEC1B
This antibody recognizes mouse C-type lectin domain family 1 member B, also known as CLEC2 or CLEC1B. CLEC2 acts as a receptor for rhodocytin, a platelet-aggregating protein found in snake venom. CLEC2 expression is not restricted to platelets and also acts as an activation receptor on neutrophils.
Tested Reactivity Ms
Tested Application FACS
Host Rat
Clonality Monoclonal
Clone 17D9
Isotype IgG2b
Target Name CLEC2 / CLEC1B
Antigen Species Mouse
Immunogen RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Conjugation FITC
Alternate Names CLEC2; C-type lectin domain family 1 member B; CLEC2B; PRO1384; C-type lectin-like receptor 2; CLEC-2; 1810061I13Rik; QDED721

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56760 Mouse CLEC1B

Swiss-port # Q9JL99 Mouse C-type lectin domain family 1 member B

Gene Symbol Clec1b
Gene Full Name C-type lectin domain family 1, member b
Background Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al., 2000 [PubMed 10671229]).[supplied by OMIM, Jan 2011]
Calculated MW 27 kDa
PTM Glycosylated.
Phosphorylated on tyrosine residue in response to rhodocytin binding.