ARG42357

anti-CLEC9A antibody [8F9] (PE)

anti-CLEC9A antibody [8F9] (PE) for Flow cytometry and Human

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [8F9] recognizes CLEC9A
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.
Host Mouse
Clonality Monoclonal
Clone 8F9
Isotype IgG2a
Target Name CLEC9A
Antigen Species Human
Immunogen RBL-2H3 cells expressing Human CLEC9A fused to an HA epitope.
Conjugation PE
Alternate Names UNQ9341; C-type lectin domain family 9 member A; CD370; DNGR-1; DNGR1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 283420 Human CLEC9A

Swiss-port # Q6UXN8 Human C-type lectin domain family 9 member A

Gene Symbol CLEC9A
Gene Full Name C-type lectin domain family 9, member A
Background CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells (Huysamen et al., 2008 [PubMed 18408006]).[supplied by OMIM, Aug 2008]
Function Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. [UniProt]
Cellular Localization Membrane; Single-pass type II membrane protein. [UniProt]
Calculated MW 27 kDa
PTM N-glycosylated. [UniProt]