ARG65434

anti-CLIC5a antibody [CLIC5-02]

anti-CLIC5a antibody [CLIC5-02] for Flow cytometry,Western blot and Human

Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [CLIC5-02] recognizes CLIC5a
Tested Reactivity Hu
Tested Application FACS, WB
Specificity The clone CLIC5-02 recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Crossreactivity with CLIC5b was not determined.
Host Mouse
Clonality Monoclonal
Clone CLIC5-02
Isotype IgG2b
Target Name CLIC5a
Antigen Species Human
Immunogen KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a
Conjugation Un-conjugated
Alternate Names Chloride intracellular channel protein 5; DFNB102; DFNB103; MST130; MSTP130

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 53405 Human CLIC5

Swiss-port # Q9NZA1 Human Chloride intracellular channel protein 5

Gene Symbol CLIC5
Gene Full Name chloride intracellular channel 5
Background CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.
Function Required for normal hearing (PubMed:24781754). It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (PubMed:15184393, PubMed:18028448, PubMed:20335315). [UniProt]
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 47 kDa