ARG41669

anti-CLTC / Clathrin heavy chain antibody

anti-CLTC / Clathrin heavy chain antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CLTC / Clathrin heavy chain
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CLTC / Clathrin heavy chain
Antigen Species Human
Immunogen Synthetic peptide of Human CLTC / Clathrin heavy chain.
Conjugation Un-conjugated
Alternate Names CHC17; CHC; Clathrin heavy chain 1; Hc; CLH-17; CLTCL2; Clathrin heavy chain on chromosome 17

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 190 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1213 Human CLTC

GeneID: 54241 Rat CLTC

GeneID: 67300 Mouse CLTC

Gene Symbol CLTC
Gene Full Name clathrin, heavy chain (Hc)
Background Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
Function Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. [UniProt]
Cellular Localization Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody
Calculated MW 192 kDa