ARG53919

anti-CLTC / Clathrin heavy chain antibody [BF-06]

anti-CLTC / Clathrin heavy chain antibody [BF-06] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Bovine,Human,Mouse,Pig,Rat

Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody

Overview

Product Description Mouse Monoclonal antibody [BF-06] recognizes CLTC / Clathrin heavy chain
Tested Reactivity Hu, Ms, Rat, Bov, Pig
Tested Application ELISA, FACS, ICC/IF, IP, WB
Specificity The clone BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.
Host Mouse
Clonality Monoclonal
Clone BF-06
Isotype IgM
Target Name CLTC / Clathrin heavy chain
Conjugation Un-conjugated
Alternate Names CHC17; CHC; Clathrin heavy chain 1; Hc; CLH-17; CLTCL2; Clathrin heavy chain on chromosome 17

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS2 - 5 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: Human blood leukocytes

Properties

Form Liquid
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95% (by SDS-PAGE)
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1213 Human CLTC

GeneID: 281080 Bovine CLTC

GeneID: 54241 Rat CLTC

Gene Symbol CLTC
Gene Full Name clathrin, heavy chain (Hc)
Background Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
Function Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30310 Endosome, Lysosome, Peroxisome Marker Antibody Panel (Catalase, Caveolin1, Clathrin heavy chain, LAMP1)
Related products:
Clathrin antibodies; Clathrin Duos / Panels; Anti-Mouse IgM secondary antibodies;
Related poster download:
Organelle Markers & Loading Control
Research Area Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody
Calculated MW 192 kDa