ARG41113

anti-CNOT8 antibody

anti-CNOT8 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CNOT8
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CNOT8
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-292 of Human CNOT8 (NP_004770.4).
Conjugation Un-conjugated
Alternate Names CCR4-associated factor 8; Caf1b; CAF1-like protein; CALIF; CAF2; CAF1; POP2; hCAF1; EC 3.1.13.4; CCR4-NOT transcription complex subunit 8; CALIFp

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver
Observed Size 34 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 69125 Mouse CNOT8

GeneID: 9337 Human CNOT8

Swiss-port # Q9D8X5 Mouse CCR4-NOT transcription complex subunit 8

Swiss-port # Q9UFF9 Human CCR4-NOT transcription complex subunit 8

Gene Symbol CNOT8
Gene Full Name CCR4-NOT transcription complex, subunit 8
Function Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity. [UniProt]
Cellular Localization Cytoplasm. Nucleus. [UniProt]
Calculated MW 34 kDa