ARG52246
anti-COBRA1 antibody [F7E4]
anti-COBRA1 antibody [F7E4] for Western blot and Human,Mouse,Rat
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [F7E4] recognizes COBRA1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | F7E4 |
| Isotype | IgG1 |
| Target Name | COBRA1 |
| Antigen Species | Human |
| Immunogen | Recombinant full length human COBRA1 purified from E. coli |
| Conjugation | Un-conjugated |
| Alternate Names | COBRA1; Negative elongation factor B; NELF-B; Cofactor of BRCA1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~62k COBRA1 protein in Western blots of neonatal Rat brain lysate. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COBRA1 |
| Gene Full Name | negative elongation factor complex member B |
| Background | COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004) |
| Research Area | Gene Regulation antibody |
| Calculated MW | 66 kDa |
