ARG52246

anti-COBRA1 antibody [F7E4]

anti-COBRA1 antibody [F7E4] for Western blot and Human,Mouse,Rat

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [F7E4] recognizes COBRA1
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone F7E4
Isotype IgG1
Target Name COBRA1
Antigen Species Human
Immunogen Recombinant full length human COBRA1 purified from E. coli
Conjugation Un-conjugated
Alternate Names COBRA1; Negative elongation factor B; NELF-B; Cofactor of BRCA1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1,000
Application Note Specific for the ~62k COBRA1 protein in Western blots of neonatal Rat brain lysate.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25920 Human NELFB

GeneID: 58202 Mouse NELFB

Swiss-port # Q8C4Y3 Mouse Negative elongation factor B

Swiss-port # Q8WX92 Human Negative elongation factor B

Gene Symbol COBRA1
Gene Full Name negative elongation factor complex member B
Background COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004)
Research Area Gene Regulation antibody
Calculated MW 66 kDa