ARG41348
anti-COP1 / RFWD2 antibody
anti-COP1 / RFWD2 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes COP1 / RFWD2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COP1 / RFWD2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 672-701 of Human COP1 / RFWD2. |
| Conjugation | Un-conjugated |
| Alternate Names | RING finger and WD repeat domain protein 2; EC 6.3.2.-; hCOP1; Constitutive photomorphogenesis protein 1 homolog; COP1; E3 ubiquitin-protein ligase RFWD2; RNF200; RING finger protein 200 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | SK-BR-3 | ||||
| Observed Size | ~ 90 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RFWD2 |
| Gene Full Name | ring finger and WD repeat domain 2, E3 ubiquitin protein ligase |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. [UniProt] |
| Cellular Localization | Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles. [UniProt] |
| Calculated MW | 80 kDa |
| PTM | Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination. [UniProt] |
