ARG42873

anti-COPS8 antibody

anti-COPS8 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes COPS8
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name COPS8
Antigen Species Human
Immunogen Synthetic peptide of Human COPS8.
Conjugation Un-conjugated
Alternate Names SGN8; COP9 signalosome complex subunit 8; Signalosome subunit 8; COP9 homolog; hCOP9; JAB1-containing signalosome subunit 8; COP9; CSN8

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:20 - 1:100
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 24 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 108679 Mouse COPS8

GeneID: 10920 Human COPS8

GeneID: 363283 Rat COPS8

Gene Symbol COPS8
Gene Full Name COP9 signalosome subunit 8
Background The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Function Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. [UniProt]
Cellular Localization Cytoplasm. Nucleus. [UniProt]
Calculated MW 23 kDa