ARG40149
anti-CORO1B phospho (Ser2) antibody
anti-CORO1B phospho (Ser2) antibody for ICC/IF,Western blot and Human,Rabbit
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CORO1B phospho (Ser2) |
|---|---|
| Tested Reactivity | Hu, Rb |
| Predict Reactivity | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody detects a ~60 kDa band corresponding to Coronin-1B on SDS-PAGE immunoblots of A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CORO1B |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide (coupled to carrier protein) around Ser2 of Human CORO1B. This sequence has high homology to the conserved site in Rat and Mouse CORO1B and in CORO1A. However, this sequence has low homology to the conserved region in other coronins. |
| Conjugation | Un-conjugated |
| Alternate Names | Coronin-1B; Coronin-2; CORONIN-2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 50% Glycerol and 1 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
GeneID: 100009415 Rabbit CORO1B |
|---|---|
| Gene Symbol | CORO1B |
| Gene Full Name | coronin, actin binding protein, 1B |
| Background | Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]).[supplied by OMIM, Mar 2008] |
| Function | Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers. [UniProt] |
| Calculated MW | 54 kDa |
| PTM | Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. [UniProt] |
