ARG56491
anti-COX2 antibody
anti-COX2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Guinea pig,Human,Monkey,Mouse,Rabbit,Rat,Sheep
Inflammation Study antibody
Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes COX2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Gpig, Mk, Rb, Sheep |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody does not react to COX-1. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COX2 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide around aa. 570-598 of Mouse / Rat COX-2. |
| Conjugation | Un-conjugated |
| Alternate Names | PHS II; Prostaglandin H2 synthase 2; PHS-2; Cyclooxygenase-2; PGHS-2; COX2; PGG/HS; COX-2; GRIPGHS; hCox-2; PGH synthase 2; Prostaglandin G/H synthase 2; Prostaglandin-endoperoxide synthase 2; EC 1.14.99.1 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, pH 7.2, 50% glycerol and 0.02% sodium azide |
| Preservative | 0.02% sodium azide |
| Stabilizer | 50% glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
GeneID: 100009248 Rabbit PTGS2 |
|---|---|
| Gene Symbol | Ptgs2 |
| Gene Full Name | prostaglandin-endoperoxide synthase 2 |
| Background | COX2: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009] |
| Function | COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis (PubMed:26859324, PubMed:27226593). Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30323 Inflammation Antibody Panel Related products: COX2 antibodies; COX2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Inflammation Study antibody |
| Calculated MW | 69 kDa |
| PTM | S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526. |
Customer's Feedback
Excellent
Review for anti-COX2 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-COX2 antibody
Application：WB
Sample：Raw264.7 (untreated or treated with LPS)
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Benzophenone and Benzoylphloroglucinol Derivatives from Hypericum sampsonii with Anti-Inflammatory Mechanism of Otogirinin A.
WB / Mouse
Hepatoma-derived growth factor participates in concanavalin A-induced hepatitis.
WB, IHC-P / Mouse, Rat
