ARG56491

anti-COX2 antibody

anti-COX2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Guinea pig,Human,Monkey,Mouse,Rabbit,Rat,Sheep

Inflammation Study antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes COX2
Tested Reactivity Hu, Ms, Rat, Gpig, Mk, Rb, Sheep
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody does not react to COX-1.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name COX2
Antigen Species Mouse
Immunogen Synthetic peptide around aa. 570-598 of Mouse / Rat COX-2.
Conjugation Un-conjugated
Alternate Names PHS II; Prostaglandin H2 synthase 2; PHS-2; Cyclooxygenase-2; PGHS-2; COX2; PGG/HS; COX-2; GRIPGHS; hCox-2; PGH synthase 2; Prostaglandin G/H synthase 2; Prostaglandin-endoperoxide synthase 2; EC 1.14.99.1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:300
IHC-P1:300
WB1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, pH 7.2, 50% glycerol and 0.02% sodium azide
Preservative 0.02% sodium azide
Stabilizer 50% glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100009248 Rabbit PTGS2

GeneID: 100135607 Guinea pig PTGS2

GeneID: 19225 Mouse PTGS2

Gene Symbol Ptgs2
Gene Full Name prostaglandin-endoperoxide synthase 2
Background COX2: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Function COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis (PubMed:26859324, PubMed:27226593). Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30323 Inflammation Antibody Panel
Related products:
COX2 antibodies; COX2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area Inflammation Study antibody
Calculated MW 69 kDa
PTM S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526.

Customer's Feedback

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Review for anti-COX2 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-COX2 antibody

Application:WB

Sample:Raw264.7 (untreated or treated with LPS)

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Benzophenone and Benzoylphloroglucinol Derivatives from Hypericum sampsonii with Anti-Inflammatory Mechanism of Otogirinin A.

WB / Mouse

Chun-Yi Huang et al.
Molecules.,  (2020)

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Hepatoma-derived growth factor participates in concanavalin A-induced hepatitis.

WB, IHC-P / Mouse, Rat

E-Ming Wang et al.
FASEB J.,  (2020)

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