ARG52252

anti-COX4 phospho (Ser58) antibody

anti-COX4 phospho (Ser58) antibody for Western blot and Mouse,Rat

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes COX4 phospho (Ser58)
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name COX4
Antigen Species Mouse
Immunogen KLH-conjugated phosphospecific peptide around Ser58 of Mouse COX IV.
Conjugation Un-conjugated
Alternate Names Cytochrome c oxidase polypeptide IV; COX4; COX4-1; COXIV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4I1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1,000
Application Note Specific for the ~17k COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12857 Mouse COX4I1

GeneID: 29445 Rat COX4I1

Swiss-port # P10888 Rat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Swiss-port # P19783 Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Gene Symbol COX4i1
Gene Full Name cytochrome c oxidase subunit IV isoform 1
Background Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Function COX4 protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt]
Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody
Calculated MW 20 kDa