ARG41033

anti-COX5A antibody

anti-COX5A antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes COX5A
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name COX5A
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-150 of Human COX5A (NP_004246.2).
Conjugation Un-conjugated
Alternate Names Cytochrome c oxidase subunit 5A, mitochondrial; VA; COX-VA; Cytochrome c oxidase polypeptide Va; COX

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse skeletal muscle
Observed Size 17 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12858 Mouse COX5A

GeneID: 252934 Rat COX5A

GeneID: 9377 Human COX5A

Gene Symbol COX5A
Gene Full Name cytochrome c oxidase subunit Va
Background Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008]
Function This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt]
Cellular Localization Mitochondrion inner membrane. [UniProt]
Calculated MW 17 kDa