ARG42992

anti-CRTC3 / TORC3 antibody

anti-CRTC3 / TORC3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CRTC3 / TORC3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CRTC3 / TORC3
Antigen Species Human
Immunogen Synthetic peptide of Human CRTC3 / TORC3.
Conjugation Un-conjugated
Alternate Names TORC3; Transducer of regulated cAMP response element-binding protein 3; TORC-3; Transducer of CREB protein 3; CREB-regulated transcription coactivator 3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:20
IHC-P1:50
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562
Observed Size ~ 73 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64784 Human CRTC3

GeneID: 70461 Mouse CRTC3

Swiss-port # Q6UUV7 Human CREB-regulated transcription coactivator 3

Swiss-port # Q91X84 Mouse CREB-regulated transcription coactivator 3

Gene Symbol CRTC3
Gene Full Name CREB regulated transcription coactivator 3
Background This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
Function Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). [UniProt]
Cellular Localization Nucleus. Cytoplasm. Note=Appears to be mainly nuclear. [UniProt]
Calculated MW 67 kDa