ARG52907
anti-CSF1R antibody [SP211]
anti-CSF1R antibody [SP211] for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP211] recognizes CSF1R |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP211 |
| Isotype | IgG |
| Target Name | CSF1R |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from human CSF-1R protein. |
| Conjugation | Un-conjugated |
| Alternate Names | CSF-1 receptor; CSF-1-R; FIM2; Macrophage colony-stimulating factor 1 receptor; Proto-oncogene c-Fms; CD115; CD antigen CD115; M-CSF-R; FMS; CSFR; C-FMS; EC 2.7.10.1; CSF-1R; HDLS |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07333 Human Macrophage colony-stimulating factor 1 receptor |
|---|---|
| Background | Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor and CD115, is a cell-surface tyrosine-protein kinase receptor for colony stimulating factor 1 (CSF1) and IL-34. It is expressed by monocytes and its progenitor or derived cells. It plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. It is up-regulated in some human tumors such as carcinoma of ovary and breast. |
| Cellular Localization | Cytoplasm, Membrane, Nucleus (cleaved form) |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 108 kDa |
| PTM | Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-561 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-561 and Tyr-809 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-699 and Tyr-923 is important for interaction with GRB2. Phosphorylation at Tyr-723 is important for interaction with PIK3R1. Phosphorylation at Tyr-708 is important for normal receptor degradation. Phosphorylation at Tyr-723 and Tyr-809 is important for interaction with PLCG2. Phosphorylation at Tyr-969 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation. Ubiquitinated. Becomes rapidly polyubiquitinated after autophosphorylation, leading to its degradation. |
