ARG63445
anti-CSK antibody
anti-CSK antibody for Western blot and Human
Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
1
Overview
| Product Description | Goat Polyclonal antibody recognizes CSK |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Specificity | Reported variants represent identical protein: NP_004374.1; NP_001120662.1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CSK |
| Antigen Species | Human |
| Immunogen | C-EQLEHIKTHELHL |
| Conjugation | Un-conjugated |
| Alternate Names | Protein-tyrosine kinase CYL; C-Src kinase; Tyrosine-protein kinase CSK; EC 2.7.10.2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CSK |
| Gene Full Name | c-src tyrosine kinase |
| Function | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. [UniProt] |
| Research Area | Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody |
| Calculated MW | 51 kDa |
| PTM | Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated. |
Customer's Feedback
Good
Review for anti-CSK antibody
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：12µl / 2ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
