ARG62966
anti-CSK antibody [CSK-04]
anti-CSK antibody [CSK-04] for Western blot,Immunoprecipitation,ICC/IF and Human,Mouse
Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
Overview
| Product Description | Mouse Monoclonal antibody [CSK-04] recognizes CSK |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IP, WB |
| Specificity | The clone CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CSK-04 |
| Isotype | IgG1 |
| Target Name | CSK |
| Antigen Species | Human |
| Immunogen | Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450. |
| Conjugation | Un-conjugated |
| Alternate Names | Protein-tyrosine kinase CYL; C-Src kinase; Tyrosine-protein kinase CSK; EC 2.7.10.2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions. IP: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | ICC/IF: HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CSK |
| Gene Full Name | c-src tyrosine kinase |
| Background | C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk. |
| Function | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30169 Src Family Protein Tyrosine Kinases Antibody Panel Related products: Csk antibodies; Csk Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Tools for studying H. pylori diseases |
| Research Area | Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody |
| Calculated MW | 51 kDa |
| PTM | Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated. |
