ARG64952

anti-CSX1 / NKX2-5 antibody

anti-CSX1 / NKX2-5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes CSX1 / NKX2-5
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CSX1 / NKX2-5
Antigen Species Human
Immunogen C-PRAYSDPDPAKDPR
Conjugation Un-conjugated
Alternate Names NKX2.5; NKX2E; Homeobox protein Nkx-2.5; CHNG5; NKX4-1; VSD3; CSX; CSX1; Homeobox protein NK-2 homolog E; HLHS2; Homeobox protein CSX; Cardiac-specific homeobox

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay - dependent
WB0.1 - 0.5 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1482 Human NKX2-5

GeneID: 18091 Mouse NKX2-5

Swiss-port # P42582 Mouse Homeobox protein Nkx-2.5

Swiss-port # P52952 Human Homeobox protein Nkx-2.5

Background This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody
Calculated MW 35 kDa