ARG56153

anti-CXCL1 / GRO alpha antibody

anti-CXCL1 / GRO alpha antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse

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Overview

Product Description Rabbit Polyclonal antibody recognizes CXCL1 / GRO alpha
Tested Reactivity Hu, Ms
Tested Application ELISA, IHC-P, Neut, WB
Specificity This antibody has been tested by ELISA to have less than 1% cross-reactivity with 50 ng/ml of human CXCL2 (GRO beta) and human CXCL3 (GRO gamma).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CXCL1 / GRO alpha
Antigen Species Human
Immunogen Recombinant Human CXCL1 protein (ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN)
Conjugation Un-conjugated
Alternate Names Growth-regulated alpha protein; SCYB1; Melanoma growth stimulatory activity; MGSA-a; GRO-alpha; GROa; NAP-3; FSP; C-X-C motif chemokine 1; Neutrophil-activating protein 3; GRO1; MGSA; IL-8 homologues (murine)

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.5 - 2.0 µg/ml (using 100 µl / well antibody solution)
IHC-P1.5 - 5 µg/ml
Neut1.5 - 3.0 µg/ml (To yield [ND50] of the biological activity of Human GRO (100 ng/ml))
WB0.1 - 0.2 µg/ml
Application Note IHC-P: Antigen Retrieval: Sodium citrate buffer (pH 6.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14825 Mouse CXCL1

GeneID: 2919 Human CXCL1

Swiss-port # P09341 Human Growth-regulated alpha protein

Swiss-port # P12850 Mouse Growth-regulated alpha protein

Gene Symbol CXCL1
Gene Full Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Background This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]
Function Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. [UniProt]
Calculated MW 11 kDa
PTM N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt]

Specific References

Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines.

Neutralize / Mouse

Rongjun Wan et al.
Aging.,  (2020)

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Therapeutic Efficacy of Subcutaneous and Intraperitoneal Injections of a Single Dose of Human Umbilical Mesenchymal Stem Cells in Acute and Chronic Colitis in a Mouse Model.

IHC-P / Mouse

Chang Yu‑Lung et al.
Journal of Medical and Biological Engineering.,  (2019)

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