ARG56663

anti-CXCL1 / GRO alpha antibody

anti-CXCL1 / GRO alpha antibody for ELISA,Neutralizing,Western blot and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CXCL1 / GRO alpha
Tested Reactivity Rat
Tested Application ELISA, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CXCL1 / GRO alpha
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat CXCL1.
(APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK)
Conjugation Un-conjugated
Alternate Names Growth-regulated alpha protein; SCYB1; Melanoma growth stimulatory activity; MGSA-a; GRO-alpha; GROa; NAP-3; FSP; 5-73; C-X-C motif chemokine 1; Neutrophil-activating protein 3; 6-73; 1-73; GRO1; MGSA; 4-73

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56773 as a detection antibody
Neut5.0 - 10.0 µg/ml (To yield [ND50] of the biological activity of Rat GRO/KC (100 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81503 Rat CXCL1

Swiss-port # P14095 Rat Growth-regulated alpha protein

Gene Symbol Cxcl1
Gene Full Name chemokine (C-X-C motif) ligand 1
Background This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]
Function Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. [UniProt]
Calculated MW 11 kDa
PTM N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt]