ARG55893

anti-CXCL12 / SDF1 antibody

Publication1

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100 μg

399.00

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Datasheet
Publication (1)

Overview

Product Description	Rabbit Polyclonal antibody recognizes CXCL12 / SDF1
Tested Reactivity	Hu, Ms, Rat
Tested Application	ELISA, IHC-Fr, IHC-P, Neut, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CXCL12 / SDF1
Antigen Species	Human
Immunogen	Recombinant Human CXCL12 / SDF1
Conjugation	Un-conjugated
Alternate Names	TPAR1; SDF1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; TLSF; PBSF; SDF-1; Intercrine reduced in hepatomas; IRH; hSDF-1; 3-72; SCYB12; hIRH; Stromal cell-derived factor 1

Application Instructions

Application Suggestion

Tested Application	Dilution
ELISA	0.5 - 2.0 µg/ml (100 µl/well)
IHC-Fr	Assay-dependent
IHC-P	0.5 µg/ml
Neut	2 - 4 µg/ml (To yield [ND50])
WB	0.1 - 0.2 µg/ml

Application Note

IHC-P: Antigen Retrieval: Sodium citrate buffer (pH 6.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Buffer	PBS (pH 7.2)
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 20315 Mouse CXCL12 GeneID: 6387 Human CXCL12 Swiss-port # P40224 Mouse Stromal cell-derived factor 1 Swiss-port # P48061 Human Stromal cell-derived factor 1
Gene Symbol	CXCL12
Gene Full Name	chemokine (C-X-C motif) ligand 12
Background	This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Function	Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [UniProt]
Calculated MW	11 kDa
PTM	Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.