ARG10829

anti-CXCR2 antibody

anti-CXCR2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes CXCR2

Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CXCR2
Antigen Species Human
Immunogen Synthetic peptide around aa. 18-39 of Human CXCR2. (EDLSNYSYSSTLPPFLLDAAPC)
Conjugation Un-conjugated
Alternate Names CD antigen CD182; High affinity interleukin-8 receptor B; CDw128b; GRO/MGSA receptor; IL-8R B; IL8RB; IL8RA; CXCR-2; IL8R2; CD182; IL-8 receptor type 2; CXC-R2; C-X-C chemokine receptor type 2; CMKAR2

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris, HCl/glycine buffer (pH 7.4-7.8), 0.02% Sodium azide, 30% Glycerol and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12765 Mouse CXCR2

GeneID: 29385 Rat CXCR2

GeneID: 3579 Human CXCR2

Gene Symbol CXCR2
Gene Full Name chemokine (C-X-C motif) receptor 2
Background The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]
Function Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. [UniProt]
Calculated MW 41 kDa
PTM Phosphorylated upon ligand binding; which is required for desensitization.