ARG54674

anti-CXCR4 antibody

anti-CXCR4 antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse

Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes CXCR4

Tested Reactivity Hu, Ms
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CXCR4
Immunogen Synthetic peptide (14 aa) within the first 50 aa of Human CXCR4.
Conjugation Un-conjugated
Alternate Names Lipopolysaccharide-associated protein 3; LAP-3; LAP3; Stromal cell-derived factor 1 receptor; Leukocyte-derived seven transmembrane domain receptor; WHIMS; NPY3R; SDF-1 receptor; Fusin; LPS-associated protein 3; HM89; HSY3RR; FB22; NPYR; CD antigen CD184; LCR1; NPYY3R; WHIM; D2S201E; C-X-C chemokine receptor type 4; LESTR; CXC-R4; CD184; NPYRL; CXCR-4

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
FACS0.1 μg/ml
ICC/IFICC: 10 μg/ml. IF: 20 μg/ml
IHC-FrAssay-Dependent. Suggest using methanol for permeabilization.
IHC-P10 μg/ml - 20 μg/ml
IP10 μg/ml
WB0.5 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa Cell Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12767 Mouse CXCR4

GeneID: 7852 Human CXCR4

Swiss-port # P61073 Human C-X-C chemokine receptor type 4

Swiss-port # P70658 Mouse C-X-C chemokine receptor type 4

Gene Symbol CXCR4
Gene Full Name chemokine (C-X-C motif) receptor 4
Background CXCR4 Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites. |
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
Calculated MW 40 kDa
PTM Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. Tyr-7 and Tyr-12 are sulfated in a sequential manner after Tyr-21 is almost fully sulfated, with the binding affinity for CXCL12/SDF-1alpha increasing with the number of sulfotyrosines present. Sulfotyrosines Tyr-7 and Tyr-12 occupy clefts on opposing CXCL12 subunits, thus bridging the CXCL12 dimer interface and promoting CXCL12 dimerization.
O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.

Customer's Feedback

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Review for anti-CXCR4 antibody

Application:WB

Sample:Bacterial infected B16-F10 cells

Sample Loading Amount:50 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:16 hours

Primary Antibody Incubation Temperature:4 ºC

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Review for anti-CXCR4 antibody

Application:WB

Sample:H1299 and A549

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses

WB / Mouse

Hao Yu et al.
Front Microbiol.,  (2023)

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