ARG55222

anti-CYC1 antibody

anti-CYC1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes CYC1

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CYC1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 142-176 (Center) of Human CYC1.
Conjugation Un-conjugated
Alternate Names Cytochrome c1, heme protein, mitochondrial; UQCR4; Cytochrome b-c1 complex subunit 4; Complex III subunit 4; Complex III subunit IV; Cytochrome c-1; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; MC3DN6

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:25
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1537 Human CYC1

GeneID: 66445 Mouse CYC1

Swiss-port # P08574 Human Cytochrome c1, heme protein, mitochondrial

Swiss-port # Q9D0M3 Mouse Cytochrome c1, heme protein, mitochondrial

Gene Symbol CYC1
Gene Full Name cytochrome c-1
Background This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6. [provided by RefSeq, Dec 2013]
Function This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain. [UniProt]
Cellular Localization Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side
Research Area Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 35 kDa
PTM Binds 1 heme group per subunit.

Customer's Feedback

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Review for anti-CYC1 antibody

Application:WB

Sample:HeLa and MCF7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC