ARG22848
anti-CYP19A1 / Aromatase antibody [H4]
anti-CYP19A1 / Aromatase antibody [H4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Baboon,Bovine,Chicken,Goat,Horse,Human,Marmoset,Mouse,Pig,Rabbit,Rat,Sheep
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [H4] recognizes CYP19A1 / Aromatase Mouse anti Human Cytochrome P450 Aromatase antibody, clone H4 recognizes a conserved epitope within cytochrome P450 aromatase (P450 arom). P450 arom plays an important role in estrogen biosynthesis and is highly conserved amongst mammals.P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots using Mouse anti Human Cytochrome P450 Aromatase antibody, clone H4 (Turner et al. 2002). |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bb, Bov, Chk, Goat, Hrs, Marmoset, Pig, Rb, Sheep |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | H4 |
| Isotype | IgG2a |
| Target Name | CYP19A1 / Aromatase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 376 - 390 of Human CYP19A1 / Aromatase. |
| Conjugation | Un-conjugated |
| Alternate Names | ARO1; CPV1; CYAR; Aromatase; Cytochrome P450 19A1; CYP19; Cytochrome P-450AROM; P-450AROM; Estrogen synthase; EC 1.14.14.14; CYPXIX; ARO |
Application Instructions
| Application Suggestion |
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| Application Note | WB: This product detects a band of approximately 55 kDa in human placental extracts. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Tissue Culture Supernatant. |
| Buffer | Tissue Culture Supernatant and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CYP19A1 |
| Gene Full Name | cytochrome P450, family 19, subfamily A, polypeptide 1 |
| Background | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Function | Catalyzes the formation of aromatic C18 estrogens from C19 androgens. [UniProt] |
| Calculated MW | 58 kDa |
| PTM | Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1). |
Specific References
Coupling of GPR30 mediated neurogenesis and protection with astroglial Aromatase-STAT3 signaling in rat hippocampus after global cerebral ischemia
IHC-Fr / Mouse
