ARG55732

anti-Calnexin antibody

anti-Calnexin antibody for Western blot,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human,Mouse,Rat,Monkey,Dog

Overview

Product Description Goat Polyclonal antibody recognizes Calnexin
Tested Reactivity Hu, Ms, Rat, Dog, Mk
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118,  rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Calnexin
Antigen Species Human
Immunogen Purified recombinant peptide around aa. 550 (C-terminus) of Human CANX
Conjugation Un-conjugated
Alternate Names P90; CNX; p90; Major histocompatibility complex class I antigen-binding protein p88; Calnexin; IP90

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50-1:500
IHC-Fr1:200-1:1000
IHC-P1:200-1:1000
WB1:500-1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12330 Mouse CANX

GeneID: 29144 Rat CANX

GeneID: 821 Human CANX

Gene Symbol CANX
Gene Full Name calnexin
Background This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
Function Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. [UniProt]
Calculated MW 68 kDa
PTM Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.
Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.