ARG64980

anti-Calnexin antibody

anti-Calnexin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Controls and Markers antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes Calnexin
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog, Pig
Tested Application ICC/IF, IHC-P, WB
Specificity Reported variants represent identical protein (NP_001019820.1, NP_001737.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Calnexin
Antigen Species Human
Immunogen C-SKTPELNLDQFHDKT
Conjugation Un-conjugated
Alternate Names P90; CNX; p90; Major histocompatibility complex class I antigen-binding protein p88; Calnexin; IP90

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
IHC-P5 µg/ml
WB0.1 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human cerebellum, Human colorectal cancer, CaCo-2 and NIH/3T3
Observed Size 90 - 100 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12330 Mouse CANX

GeneID: 821 Human CANX

Swiss-port # P27824 Human Calnexin

Swiss-port # P35564 Mouse Calnexin

Background This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
Research Area Controls and Markers antibody; Neuroscience antibody
Calculated MW 68 kDa
PTM Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.
Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.