ARG54735
anti-Calpain 1 antibody
anti-Calpain 1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes Calpain 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1376CT809.24.85 |
| Isotype | IgG1 Kappa |
| Target Name | Calpain 1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide from Human CAPN1 protein (NP_001185797.1). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.4.22.52; CANP1; Calpain-1 large subunit; Calpain-1 catalytic subunit; Calpain mu-type; muCANP; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; CANP 1; CANP; muCL; CANPL1; Calcium-activated neutral proteinase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Protein G purified |
|---|---|
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CAPN1 |
| Gene Full Name | calpain 1, (mu/I) large subunit |
| Background | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [From Uniprot] |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding |
| Highlight | Related products: Calpain1 antibodies; Related poster download: The NF-kappa B Pathways.pdf |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 82 kDa |
| PTM | Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. |
