ARG22220

anti-Calreticulin antibody

anti-Calreticulin antibody for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,Flow cytometry and Dog,Human,Monkey,Mouse,Rat,Bovine,Chicken,Guinea pig,Hamster,Pig,Rabbit,Sheep

Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Calreticulin
Tested Reactivity Hu, Ms, Rat, Bov, Chk, Dog, Gpig, Hm, Mk, Pig, Rb, Sheep
Tested Application FACS, ICC/IF, IHC, IP, WB
Specificity Detects ~63kDa.
Host Rabbit
Clonality Polyclonal
Target Name Calreticulin
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide from Human calreticulin (with a cysteine residue added)
Conjugation Un-conjugated
Alternate Names HACBP; cC1qR; CRT; ERp60; Calregulin; Calreticulin; CRP55; Endoplasmic reticulum resident protein 60; HEL-S-99n; RO; SSA; grp60

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:100
IHC1:100
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100009050 Rabbit CALR

GeneID: 100381266 Pig CALR

GeneID: 12317 Mouse CALR

Gene Symbol CALR
Gene Full Name calreticulin
Background Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]
Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). [UniProt]
Cellular Localization Cytoplasm, Cell Surface, Endoplasmic Reticulum, Endoplasmic reticulum lumen, Extracellular Matrix, Sarcoplasmic Reticulum, Sarcoplasmic Reticulum Lumen
Research Area Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody
Calculated MW 48 kDa