ARG55123
anti-Calreticulin antibody
anti-Calreticulin antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Calreticulin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Calreticulin |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human Calreticulin (Swiss: P27797) |
| Conjugation | Un-conjugated |
| Alternate Names | HACBP; cC1qR; CRT; ERp60; Calregulin; Calreticulin; CRP55; Endoplasmic reticulum resident protein 60; HEL-S-99n; RO; SSA; grp60 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CALR |
| Gene Full Name | calreticulin |
| Background | Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008] |
| Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30316 ER Marker Antibody Duo Related products: Calreticulin antibodies; Calreticulin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody |
| Calculated MW | 48 kDa |
Customer's Feedback
Excellent
Review for anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
Review for anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
Review for anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：Methanol / Acetone 1:1 ratio
Fixation Time：20 min
Fixation Temperature：-20 ºC
Antigen Retrieval Buffer：N/A
Antigen Retrieval Time：N/A
Antigen Retrieval Temperature：N/A ºC
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：1 hour
Primary Antibody Incubation Temperature：RT ºC
Excellent
Review for anti-Calreticulin antibody
Application：WB
Sample：NIH-3T3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.
WB / Human
