ARG10703

anti-Calretinin antibody [6A9]

anti-Calretinin antibody [6A9] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow

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Overview

Product Description

Mouse Monoclonal antibody [6A9] recognizes Calretinin

Tested Reactivity Hu, Ms, Rat, Cow
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 6A9
Isotype IgA
Target Name Calretinin
Antigen Species Human
Immunogen Full-length recombinant Human protein.
Conjugation Un-conjugated
Alternate Names CAB29; CR; CAL2; 29 kDa calbindin; Calretinin

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000 - 1:5000
IHC-Fr1:2000 - 1:5000
WB1:2000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117059 Rat CALB2

GeneID: 12308 Mouse CALB2

GeneID: 794 Human CALB2

Gene Symbol CALB2
Gene Full Name calbindin 2
Background This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Function Calretinin is a calcium-binding protein which is abundant in auditory neurons. [UniProt]
Calculated MW 32 kDa

Customer's Feedback

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Review for anti-Calretinin antibody [6A9]

Application:WB

Sample:Rat brain

Sample Loading Amount:15 µg

Primary Antibody Dilution Factor:1:2500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Calretinin antibody [6A9]

Application:WB

Sample:Mouse Cerebellum

Sample Loading Amount:15 µg

Primary Antibody Dilution Factor:1:2500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC