ARG62381

anti-Cardiac Troponin I antibody [2D5]

anti-Cardiac Troponin I antibody [2D5] for ELISA,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [2D5] recognizes Cardiac Troponin I
Tested Reactivity Hu
Tested Application ELISA, IHC-P, IP, WB
Specificity Reacts with subunit I of the troponin complex in human myocardium,
Host Mouse
Clonality Monoclonal
Clone 2D5
Isotype IgG1
Target Name Cardiac Troponin I
Antigen Species Human
Immunogen Purified human cardiac troponin I
Conjugation Un-conjugated
Alternate Names RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7

Application Instructions

Application Note ELISA: 1/500 - 1/1000.
IHC-P: 1/25 - 1/50.
WB: 1/50 - 1/100.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A purified
Buffer PBS (pH 7.2), 0.2% BSA and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 0.2% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7137 Human TNNI3

Swiss-port # P19429 Human Troponin I, cardiac muscle

Gene Symbol TNNI3
Gene Full Name troponin I type 3 (cardiac)
Background Troponin I is the inhibitory subunit of troponin, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 24 kDa
PTM Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

Clone References

Spontaneous cardiomyopathy in cynomolgus monkeys (Macaca fascicularis).

IHC / Primates

Zabka TS et al.
Toxicol Pathol.,  (2009)

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Apoptosis predominates in nonmyocytes in heart failure.

IHC / Primates

Park M et al.
Am J Physiol Heart Circ Physiol.,  (2009)

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