ARG52454

anti-Cardiac Troponin I phospho (Ser150) antibody

anti-Cardiac Troponin I phospho (Ser150) antibody for Western blot and Mouse,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Cardiac Troponin I phospho (Ser150)
Tested Reactivity Ms, Rat
Predict Reactivity Hu, NHuPrm
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cardiac Troponin I
Antigen Species Mouse
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH
Conjugation Un-conjugated
Alternate Names RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note Specific for the ~25k cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21954 Mouse TNNI3

GeneID: 29248 Rat TNNI3

Swiss-port # P23693 Rat Troponin I, cardiac muscle

Swiss-port # P48787 Mouse Troponin I, cardiac muscle

Gene Symbol TNNI3
Gene Full Name troponin I, cardiac 3
Background Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 24 kDa
PTM Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).