ARG65648

anti-Cardiac Troponin T antibody

anti-Cardiac Troponin T antibody for Western blot and Human,Mouse,Rat,Pig

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Cardiac Troponin T
Tested Reactivity Hu, Ms, Rat, Pig
Predict Reactivity Cat, Cow, Dog
Tested Application WB
Specificity This antibody is expected to recognize all reported isoforms (NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1).
Host Goat
Clonality Polyclonal
Target Name Cardiac Troponin T
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human Cardiac Troponin T (C-RNRINDNQKVSKT)
Conjugation Un-conjugated
Alternate Names RCM3; CMPD2; LVNC6; TnTC; TnTc; CMH2; cTnT; Cardiac muscle troponin T; CMD1D; Troponin T, cardiac muscle

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21956 Mouse TNNT2

GeneID: 24837 Rat TNNT2

GeneID: 7139 Human TNNT2

Gene Symbol TNNT2
Gene Full Name troponin T type 2 (cardiac)
Background The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 35.6 kDa (NP_000355.2)
PTM Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.